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Table 5 Subcutaneous fat mRNA abundance at 4 days post-hatch

From: The effects of dietary macronutrient composition on lipid metabolism-associated factor gene expression in the adipose tissue of chickens are influenced by fasting and refeeding

Effect1

AGPAT9

FABP4

C/EBPα

C/EBPβ

KLF7

PPARγ

MGLL

SREBP1

 Diet

  HC

0.44

1.06a

0.35

1.77

1.31

0.45

2.60

0.51

  HF

0.36

1.00ab

0.32

1.40

1.32

0.50

2.21

0.43

  HP

0.36

0.84b

0.32

1.33

1.19

0.39

2.31

0.41

  SEM

0.04

0.06

0.02

0.18

0.09

0.04

0.27

0.04

   P-value

0.23

0.02

0.37

0.21

0.66

0.27

0.55

0.10

  Fed

0.57a

1.03

0.51a

1.00b

1.28b

0.73a

2.42

0.67a

  Fasted

0.31b

0.96

0.16c

2.26a

1.59a

0.32b

2.54

0.39b

  Refed

0.31b

0.91

0.33b

1.18b

0.97c

0.32b

2.18

0.33b

  SEM

0.04

0.05

0.02

0.17

0.09

0.04

0.26

0.04

   P-value

0.0001

0.35

0.0001

0.0001

0.0001

0.0001

0.57

0.0001

  D × T

0.13

0.22

0.003

0.31

0.77

0.21

0.67

0.06

Effect1

GATA2

ATGL

PLN1

CGI-58

ACADL

NPY

NPYR2

LPL

 Diet

  HC

0.97

1.41

0.89

1.24

0.70

0.91

0.69

1.21

  HF

1.05

1.42

0.77

0.70

0.56

0.64

0.66

1.41

  HP

0.86

1.36

0.87

0.96

0.49

1.09

0.61

1.64

  SEM

0.07

0.17

0.13

0.19

0.15

0.26

0.05

0.19

   P-value

0.11

0.96

0.76

0.15

0.57

0.46

0.48

0.30

Treatment

 Fed

1.10

1.50

0.79

1.20

0.72

1.35

0.89a

1.55

 Fasted

0.90

1.38

1.00

0.93

0.76

0.85

0.56b

1.25

 Refed

0.89

1.31

0.74

0.77

0.28

0.44

0.50b

1.47

 SEM

0.06

0.17

0.13

0.19

0.15

0.26

0.05

0.19

  P-value

0.08

0.76

0.30

0.29

0.03

0.07

0.0001

0.50

 D × T

0.26

0.76

0.56

0.51

0.14

0.76

0.03

0.37

  1. 1Values represent least squares means and pooled standard errors of the means with associated P-values for each effect. D × T: interaction between diet (D) and treatment (T). Different superscipts within an effect for each gene are significantly different at P < 0.05, Tukey’s test. Abbreviations: 1-acylglycerol-3-phosphate O-acyltransferase 9: AGPAT9; Fatty acid binding protein 4: FABP4; CCAAT/enhancer-binding protein alpha and beta: C/EBPα and C/EBPβ, respectively; Krüppel-like factor 7: KLF7; Peroxisome proliferator-activated receptor gamma: PPARγ; Monoglyceride lipase: MGLL; Sterol regulatory element-binding transcription factor 1: SREBP1; GATA-binding protein 2: GATA2; Adipose triglyceride lipase: ATGL; Perilipin 1: PLN1; Comparative gene identification-58: CGI-58; Acyl-CoA dehydrogenase, long chain: ACADL; Neuropeptide Y: NPY; NPY receptor 2: NPYR2; Lipoprotein lipase: LPL